LINC00094靶向miR-19b对非小细胞肺癌增殖、侵袭的抑制作用及其分子机制

doi:10.3969/j.issn.2095-1264.2022.06.12

首页 > 过刊浏览>2022年第6期 >759-766. DOI:10.3969/j.issn.2095-1264.2022.06.12

LINC00094靶向miR-19b对非小细胞肺癌增殖、侵袭的抑制作用及其分子机制
DOI:
                        10.3969/j.issn.2095-1264.2022.06.12
                    
作者:
                        
                        
                    
作者单位:1.临沂市肿瘤医院，胸外科，山东 临沂，276300;2.临沂市肿瘤医院，手术室，山东 临沂，276300
作者简介:邢加强，男，硕士，副主任医师，研究方向：肺癌。
通讯作者:辛本强，男，副主任医师，研究方向：胸外科疾病。
中图分类号:R734.2
基金项目:

Inhibitory effects of LINC00094/miR-19b axis on proliferation and invasion of non-small cell lung cancer and its mechanism

Author:

Affiliation:

1.Thoracic Surgery Department, Linyi Cancer Hospital, Linyi, 276300, Shandong, China;2.Operation Room, Linyi Cancer Hospital, Linyi, 276300, Shandong, China

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摘要:

目的探讨LINC00094对非小细胞肺癌A549细胞增殖、凋亡、迁移、侵袭的影响及其作用机制。方法将pcDNA3.1、pcDNA3.1-LINC00094、si-NC、si-LINC00094转染至非小细胞肺癌A549细胞中；将pcDNA3.1-LINC00094分别与miR-NC、miR-19b转染至A549细胞中，分别记为pcDNA3.1-LINC00094+miR-NC组、pcDNA3.1-LINC00094+miR-19b组。采用实时荧光定量PCR检测LINC00094和miR-19b表达水平；采用蛋白质印迹法检测细胞周期蛋白和凋亡蛋白表达水平；采用四甲基偶氮唑盐比色法、流式细胞术和Transwell分别检测细胞增殖、凋亡、迁移和侵袭能力；双荧光素酶报告实验检测LINC00094与miR-19b的靶向关系。结果 LINC00094在肺癌组织中低表达。过表达LINC00094可降低A549细胞的生存率，并抑制细胞迁移、侵袭（P<0.05），且伴有相关蛋白表达水平的改变（P<0.05）。LINC00094靶向调控miR-19b，过表达miR-19b可逆转LINC00094对A549细胞增殖、迁移、侵袭的抑制作用和对细胞凋亡的促进作用。结论 LINC00094可靶向抑制miR-19b的表达，从而抑制A549细胞的增殖、迁移和侵袭。

Abstract:

Objective To investigate the effects of LINC00094 on the proliferation, apoptosis, migration and invasion of non-small cell lung cancer A549 cells and its mechanism.Methods pcDNA3.1, pcDNA3.1-LINC00094, si-NC and si-LINC00094 were transfected into A549 cells, respectively. pcDNA3.1-LINC00094 was transfected into A549 cells respectively with miR-NC and miR-19b. Real-time quantitative PCR was used to detect the expressions of LINC00094 and miR-19b; Western blotting was adopted to detect cyclin- and apoptosis-related protein markers; methyl thiazolyl tetrazolium (MTT), flow cytometry and Transwell assays were utilized to test the viability, apoptosis, invasion and migration of A549 cells, respectively. Dual luciferase reporter assay was applied for the detection of the targeting relationship between LINC00094 and miR-19b.Results The expression of LINC00094 was low in lung cancer tissues. After overexpression of LINC00094, the cell survival rate was significantly decreased, cell apoptosis was notably increased, and the number of cell migration and invasion was significantly decreased (P<0.05), which were accompanied with the changes of expression levels of related proteins (P<0.05). LINC00094 targeted to regulate the expression of miR-19b, and overexpression of miR-19b reversed the effects of LINC00094 on the proliferation, apoptosis, migration and invasion of A549 cells.Conclusion LINC00094 inhibits the survival, migration and invasion of A549 cells through suppressing miR-19b expression in a targeted manner.