miR-552通过PTEN/AKT信号通路促进非小细胞肺癌A549细胞的恶性生物学行为

doi:10.3969/j.issn.2095-1264.2022.02.09

首页 > 过刊浏览>2022年第2期 >197-203. DOI:10.3969/j.issn.2095-1264.2022.02.09

miR-552通过PTEN/AKT信号通路促进非小细胞肺癌A549细胞的恶性生物学行为
DOI:
                        10.3969/j.issn.2095-1264.2022.02.09
                    
作者:
                        
                        
                    
作者单位:1.上海交通大学医学院附属新华医院崇明分院 检验科，上海，202150;2.浦东新区人民医院 检验科， 上海，201200;3.上海市第六人民医院东院 检验科，上海，201300
作者简介:李玉珍，女，主管检验师，研究方向：临床生化和肿瘤免疫检测研究。
通讯作者:张莉，女，主任技师，研究方向：临床免疫检测和实验室管理方面研究。
中图分类号:R734.2
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miR-552 promotes malignant biological behavior of human non-small cell lung cancer A549 cells via the PTEN/AKT signaling pathway

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Affiliation:

1.Department of Laboratory, Chongming Branch, the Xinhua Hospital Affiliated to the School of Medicine of Shanghai Jiaotong University, Shanghai, 202150, China;2.Department of Laboratory, Pudong New Area People's Hospital, Shanghai, 201299, China;3.Department of Laboratory, Shanghai Sixth People's Hospital East, Shanghai, 201300, China

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摘要:

目的探讨miR-552通过调控PTEN/AKT信号通路促进人非小细胞肺癌A549细胞的恶性生物学行为及其相关机制。方法通过qRT-PCR检测人肺癌组织样本和人非小细胞肺癌A549细胞系中miR-552、PTEN mRNA的表达情况；通过Western blotting检测PTEN、AKT、p-AKT蛋白的表达情况；通过CCK-8检测miR-552表达对A549细胞增殖能力的影响；通过Transwell小室检测miR-552表达对A549细胞迁移和侵袭能力的影响；通过流式细胞术检测miR-552表达对A549细胞凋亡能力的影响。结果 miR-552在肺癌组织和A549细胞中的表达显著上调。过表达miR-552可显著促进A549细胞的增殖、迁移和侵袭，并抑制细胞凋亡，而抑制其表达则结果相反。与癌旁组织相比，肺癌组织中PTEN表达显著下调。过表达miR-552可下调PTEN蛋白表达，上调p-AKT蛋白表达，对AKT蛋白无影响，而抑制其表达则结果相反。结论 miR-552可能通过PTEN/AKT信号通路促进人非小细胞肺癌A549细胞的恶性生物学行为。

Abstract:

Objective To investigate the effects of miR-552 expression on the malignant biological behavior of human non-small cell lung cancer A549 cells by PTEN/AKT signaling pathway.Methods The expression of miR-552 and PTEN mRNA in human lung cancer tissues and non-small cell lung cancer A549 cells was detected by qRT-PCR. The expressions of relative proteins (PTEN, AKT, p-AKT) were detected by Western blotting. The proliferation of A549 cells was determined by CCK-8 assay, and the migration and invasion were detected by Transwell assay. The apoptosis of A549 cells was analyzed via flow cytometry.Results The expression of miR-552 was dramatically up-regulated in tumor tissues and A549 cells. The over-expressed miR-552 significantly promoted the proliferation, migration and invasion of A549 cells, and inhibited the apoptosis of A549 cells, while the inhibited expression of miR-552 showed opposite effects on A549 cells. Compared with the paracancerous normal tissues, the expression of miR-552 was dramatically down-regulated in the lung cancer tissues. In addition, the over-expression of miR-552 decreased the expression of PTEN, and increased the expression of p-AKT protein without affecting AKT protein, whereas the inhibited expression of miR-552 showed the opposite effects.Conclusion miR-552 may promote the malignant biological behavior of human non-small cell lung cancer A549 cells by regulating the PTEN/AKT signaling pathway.