The research progress of Chinese medicine monomer on the regulation of ovarian cancer signaling pathway in recent years is reviewed here. Traditional Chinese medicine monomer can regulate multiple signaling pathways of ovarian cancer. According to their mechanism of action, there are mainly seven types of regulation: the regulation of PI3K/AKT signaling pathway (such as by icariin, cepharanthine, naringetol, etc.), the regulation of JAK/STAT signaling pathway (such as by resveratrol, quercetin, curcumol, etc.), the regulation of MAPK signaling pathway (such as by diosgenin, gentiopicrin, dihydromyricetin, etc.), the regulation of Wnt/β-catenin signaling pathway (such as by hydroxysafflor yellow A, paeonol, apigenin, etc.), the regulation of Notch signaling pathway (such as by dihydroartemisinin, kaempferol, curcumin, etc.), the regulation of NF-κB signaling pathways (such as by ginsenoside Rg1, dihydrotanshinone Ⅰ, ampelopsin, etc.), and the regulation of TLR4/MyD88 signaling pathways (such as by sulforaphane, atractylenolide Ⅰ, curcumin, etc.). These regulations can inhibit the proliferation of ovarian cancer cells, induce cell apoptosis, block cell cycle, inhibit cell migration and invasion ability, so that can play a corresponding anti-tumor role.