海南省肿瘤医院 普外科，海南 海口，570000
Hainan Cancer Hospital, Haikou, 570000, Hainan, China
目的 分析结肠癌患者血清外泌体miR-320的表达及其临床意义。方法 选取2018年4月至2020年4月我院收治的50例结肠癌患者纳入研究，另选取50例体检健康者纳入对照组，收集两组的血清样本，采用RT-qPCR法检测血清外泌体中miR-320的表达，并分析其对结肠癌的诊断价值。结果 纯化的血清样品中存在大量外泌体颗粒，其特性与其他相关研究报道一致。结肠癌患者血清外泌体中miR-320的相对表达量明显低于健康对照组［（0.69±0.23） vs. （1.53±0.48）， t=11.16， P<0.001］。血清外泌体miR-320诊断结肠癌的曲线下面积为0.871（95% CI： 0.596~0.925），诊断敏感度为90.73%，特异度为82.43%。结论 结肠癌患者血清外泌体中miR-320表达下调，可能作为结肠癌早期诊断的评价指标。
Objective To analyze the expression and clinical significance of serum exosome miR-320 in patients with colon cancer.Methods A total of 50 patients with colon cancer admitted in our hospital between April 2018 and April 2020 were included in the study, and 50 healthy patients were included in the control group. The serum samples were collected from the two groups, and the expression of miR-320 in serum exosomes was detected by RT-qPCR method, and the diagnostic value of colon cancer was analyzed.Results There were a large number of exosome particles in the purified serum samples, and its characteristics were consistent with those reported in other relevant research. The relative expression of miR-320 in serum exosomes of patients with colon cancer was significantly lower than that of healthy controls [(0.69±0.23) vs. (1.53±0.48), t=11.16, P<0.001]. The area under the curve for serum exosome miR-320 in the diagnosis of colon cancer was 0.871 (95% CI: 0.596~0.925), with the diagnostic sensitivity of 90.73%, and the specificity of 82.43%.Conclusion The expression of miR-320 was down-regulated in the serum exosomes of patients with colon cancer. The miR-320 in serum exosome may become an evaluation indicator for early diagnosis of colon cancer.
王治帮,关道宏,谢彝忠.结肠癌患者血清外泌体miR-320的表达及其临床意义[J].肿瘤药学,2023,13(4):461-465 ( in Chinese)复制