1.江阴市妇幼保健院，江苏 无锡，214400;2.南京市第二医院，江苏 南京，210003
1.Jiangyin City Maternal and Child Health Hospital, Wuxi, 214400, Jiangsu, China;2.Nanjing Second Hospital, Nanjing, 210003, Jiangsu, China
目的 研究circ-E2F3通过抑制miR-296-5p影响宫颈癌进展的作用机制。方法 采用qRT-PCR检测宫颈癌组织和癌旁组织中circ-E2F3的表达量，筛选宫颈癌细胞系，通过qRT-PCR检测宫颈癌细胞系和正常宫颈黏膜上皮细胞中circ-E2F3的表达量。采用CCK-8检测细胞活力，通过检索数据库筛查circ-E2F3可能的分子机制，并通过双荧光素酶分析、qRT-PCR等方法证实其调控机制。结果 circ-E2F3在宫颈癌组织和宫颈癌细胞系CaSki中表达上调。体外实验证实circ-E2F3能促进宫颈癌细胞增殖；体内实验证实circ-E2F3通过抑制miR-296-5p促进肿瘤细胞生长。结论 circ-E2F3通过抑制miR-296-5p在宫颈癌的进展中发挥重要作用，高表达的circ-E2F3可能是判断宫颈癌进展的重要指标。
Objective To study the effect of circ-E2F3 on the progression of cervical cancer by inhibiting miR-296-5p.Methods The expression levels of circ-E2F3 in cervical cancer tissues and adjacent tissues were detected by qRT-PCR, and cervical cancer cell lines were then screened. The expression levels of circ-E2F3 expression in cervical cancer cell lines and normal cervical mucosal epithelial cells were detected by qRT-PCR. Cell viability was detected by CCK-8. The possible molecular mechanism of circ-E2F3 was screened by searching the database, and its regulatory mechanism was confirmed by dual luciferase analysis and qRT-PCR.Results The expression of circ-E2F3 was up-regulated in cervical cancer tissues and cervical cancer cell line CaSki. In vitro experiment found that circ-E2F3 can promote the proliferation of cervical cancer cells. circ-E2F3 promoted the tumor growth in vivo by inhibiting miR-296-5p.Conclusion circ-E2F3 played an important role in the progress of cervical cancer by inhibiting miR-296-5p. Highly-expressed circ-E2F3 may be an important indicator to predict the progress of cervical cancer.
缪华珍,刘敏. circ-E2F3促进宫颈癌细胞增殖的机制研究[J].肿瘤药学,2023,13(4):443-448 ( in Chinese)复制